Fig. 1. Effect of EtBr on growth of Dictyostelium discoideum Ax-2 cells. Various concentrations of EtBr were added to exponentially growing cells (2x105 cells/ml) in axenic growth medium (PS medium), followed by cell counts under a haemocytometer. (
) control (non-EtBr-treated cells); (
) cells treated with 10 µg/ml of EtBr, (
) 20 µg/ml of EtBr, (
) 30 µg/ml of EtBr, (
) 40 µg/ml of EtBr and (
) 50 µg/ml of EtBr. Similar results were obtained by cell counts in three independent experiments.