Fig. 2. Stainings of Ax-2 cells treated with 30 µg/ml of EtBr for 40 hours and nontreated cells with DAPI (A,C) and MitoTracker Orange (B,D). In nontreated Ax-2 cells, DAPI stains are noticed in nuclei (A, arrowheads) and mitochondria as granular structures (A, arrows). In EtBr-treated cells, however, the DAPI-staining of mitochondria is almost vanished, although the staining of nuclei is retained (C). However, rather stronger staining by MitoTracker Orange is observed in a limited number of mitochondria (D, arrows) contained in EtBr-treated cells compared with nontreated cells (B) at the vegetative growth phase. Bars, 10 µm.