(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. MURF-2 colocalizes with a portion of microtubules and with assembling myofibrils in primary cultures of skeletal myocytes. Myofibrillogenesis in skeletal myogenic cells was analyzed by costaining myotubes for {alpha}-actinin (d,f and data not shown). MURF-2 staining was mainly diffuse in the cytoplasm throughout the culture period (a,c,e). In addition, some MURF-2 staining was detected colocalized with {alpha}-tubulin staining early in culture (a,b; merged inset in b; red is MURF-2 staining and green is tubulin; double arrows point to colocalization). Later during myofibrillogenesis, MURF-2 staining was detected at the M-line region, although in relatively few myofibrils (c,d; merged image in d; MURF-2 staining in red, {alpha}-actinin staining is green; double arrows point to myofibrils). In mature myotubes, MURF-2 staining also could be detected along the length of some myofibrils (e,f; double arrowheads). Bar, 10 µm.