Fig. 1. Binding of Fer to p120ctn. (A) Full-length and GST fusion constructs of Fer used in the in vitro binding assay. CC1, CC2 and CC3 correspond to coiled-coil domains. (B) Recombinant GST-Fer peptides were purified by affinity chromatography on glutathione-agarose and incubated with biotin-conjugated CBP-p120ctn immobilized on streptavidin-coated wells. Bound protein was determined by ELISA using anti-GST antibody and the appropriate HRP-conjugated secondary antibody. (C) Dose response of binding of the GST-Fer constructs to immobilized p120ctn. Immobilized p120ctn was incubated with increasing concentrations of GST-Fer constructs 1-7 and bound protein determined as described in B.