Fig. 7. Effect of FerP on cells expressing mutant ß-catenin. GST, GST/wild-type-ß-catenin, GST/Y142F-ß-catenin or GST/Y654F-ß-catenin were introduced into E8 chick neural retina cells using BioPorter and the cells treated with FerP or FerCo peptides for 1 hour. (A) Cells were lysed in nonionic detergent, immunoprecipitated with anti-cadherin antibody and immunoblotted with the indicated antibody. (B) Intact cells were aliquoted into wells precoated with Fc-N-cad and assayed for adhesion. Adhesion is expressed as the percentage of adhesion among cells treated with GST and CoP.