Fig. 2. Real-time RT-PCR analysis of testicular resistin mRNA levels throughout post-natal development and in aging. Total RNA samples were obtained from 5-day, 15-day, 30-day, 60-day, 90-day, and 17-month-old rats. Quantitative data in terms of resistin expression levels were normalized to those of the internal control RP-L19. Values are the mean±s.e.m. of at least four determinations. a,b and c denote groups that are statistically different (P<0.01; ANOVA followed by Tukey's test).