Fig. 4. Effects of hypophysectomy (HPX) and gonadotropin replacement on testicular expression of resistin. (Left panels) A representative RT-PCR assay of resistin mRNA levels in testes from long-term (4-week) HPX rats, with or without replacement of hCG (10 IU/rat/24 hours for 7 days) or FSH (7.5 IU/rat/24 hours for 7 days). Semi-quantitative values are the mean±s.e.m. of at least four independent determ inations. a,b and c denote groups that are statistically different (P<0.01; ANOVA followed by Tukey's test). (Right panels) Representative photomicrographs of resistin immunoreactivity in testis tissue from HPX rats, with or without replacement of hCG. (A) A representative control section. (B,C) Long-term HPX resulted in clear-cut suppression of resistin immunoreactivity (B), whereas hCG replacement partially restored this signal (C). Scale bar: 20 µm.