Fig. 4. (A) IPCR profiles of irradiated mdx nu/nu TA muscles of three mice injected with 5x10⁵ isolated fibre MPC (population C). Three weeks after MPC transplantation, the right TA muscles were damaged by notexin injection and muscles were examined seven days later. Profiles are shown of infected cells (population C) at passage four on the day of transplantation (p4), at passage eight (p8) and of muscles of three mice (1l-3r) four weeks after cell transplantation. R refers to the TA of the right leg and l to the TA of the left leg. C refers to the constant band and arrows 1-12 refer to the IPCR products present either in the cell preparations, or in the injected muscles. (B) IPCR profiles of five individual clones (1-5); four ß-gal-positive clones (from population C) and one ß-gal-negative clone (from population B). (C) IPCR profile of the mixed clones before injection (p1), following four weeks in culture (p7) and muscles of three mice that had been transplanted with 5x10⁵ cells total (1x10⁵ cells of each clone). Three weeks after MPC transplantation, right TA muscles were damaged by notexin injection and muscles were analysed seven days later. L and r refer to the left and right TA muscles. Arrows show the constant band (C) and products of 900, 750, 500 and 450 bp (Table 2) that are present within the clones and injected muscles (1l-3r). (D) IPCR profiles of mdx nu/nu TA muscles of three mice injected with 5x10⁵ isolated fibre MPCs (population C). Profiles are shown of infected cells (population C) at passage five on the day of transplantation (p5), at passage 11 (p11) and of muscles of three mice (1l-3r) three weeks after cell transplantation. R refers to the irradiated TA of the right leg and l to the non-irradiated TA of the left leg.