Fig. 4. Overexpression of truncated forms of myosin IB. (A) The E. histolytica myosin IB heavy chain domains and the truncated forms of the protein used in this study. (B) The same amount of total cell lysates from amoebae transfected with the indicated constructs were obtained and the expression level of myosin IB proteins was quantified by immunoblot. The black arrow indicates the endogenous myosin IB (129 kDa) and the asterisks indicate the truncated forms: Myo-tail+, 47 kDa; SH3, 124 kDa; TH2, 125 kDa. The different truncated forms of myoIB were overexpressed at equivalent levels to the full-length protein in MyoIB+ strain and about three times more than in the wild-type cells.