(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 10. Expression of claudins in cultured RPE. RPE was isolated from E7 or E14 embryos and cultured for 9 days with the various media in the apical and basal medium chambers. Total RNA was isolated and analyzed using real-time RT-PCR (A-C). (A) Basal SF3 and apical rcSF3. (B) Basal SF2 and apical SF3 or rcSF3, as indicated. (C) Basal CEF conditioned medium and apical SF3 or rcSF3, as indicated. Data, with standard error, represent the average of 3-5 determinations. *Significant effect of rcSF3 (P<0.05); {dagger}Significant difference between E14 and E7 RPE that were maintained in the same culture conditions (P<0.05). (D) Cultures stained for the indicated claudin were viewed en face by epifluorescence microscopy. Double staining with ZO-1 (not shown) was used to adjust the focal plane to the junctional region of the lateral membranes. Bar, 10 µm.