Fig. 9. Quantification of the effects of CEF and rcSF3 media on cultures of E14 RPE. Strand number (A) and depth (B) were determined for cultures depicted in Fig. 8. The basal medium chamber contained CEF medium, but the apical chamber contained either SF3 () or rcSF3 (). A continuous network of junctional strands induced by CEF, with some regions exhibiting very high number of parallel strands with great depth. The effect of rcSF3 was to increase strand number and depth. Curiously, there was a bimodal distribution of junctional strands in each growth condition. Nonetheless, the more prominent mode in rcSF3 resembled E14 RPE in vivo.