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Fig. 5. Hop1 and Mek1 localization to Rec10 LEs shown by immunostaining of Rec10, Hop1 and Mek1-HA. Regions where Hop1 or Mek1 (green) colocalize with Rec10 (red) appear orange in the merge image. Chromatin is stained blue with DAPI. The images show Hop1 localization to: (a) class Ib LEs of the wild type (WT); (b) residual LEs in the rec10-109 mutant; (c) LEs in a rec12-152 mutant strain; (d) aberrant LEs in the rec8{Delta} mutant; and (e) LEs in a mek1{Delta} strain. Some short LEs are lacking Hop1 and Hop1 localization to longer LEs is discontinuous. Mek1 forms dots on short LEs in class I nuclei (f) but covers most parts of LE bundles in (rare) class IIb nuclei (g). Scale bar, 5 µm.