Fig. 2. NHE3-EGFP expression in transiently transfected OK cells. Images were collected 48 hours after transfection with NHE3-EGFP, during which time the cells were incubated with serum-free media. Fluorescence micrographs were taken with a 100x objective. NHE3-EGFP localized in three pools in OK cells: in the juxtanuclear (JN) area (as previously described) (D'Souza et al., 1998; Akhter et al., 2002) (A), in the microvilli of the apical plasma membrane above the JN or in a non-JN area (B). Bar, 10 µm. (C) XZ reconstruction showing NHE3-EGFP distribution in apical membrane and JN area. (D) The three pools of NHE3-EGFP in OK cells. A and B were oversaturated on purpose to show both microvillus and intracellular NHE3 locations.