Fig. 6. Decreasing surface NHE3 by inhibiting phosphoinositide 3-kinase did not alter fluorescence recovery of non-JN NHE3-EGFP remaining in the apical surface. OK/NHE3-EGFP cells were incubated with 50 µM LY294002 for 30 minutes at 37°C, then fluorescence recovery measurements were performed with analysis of apical non-JN NHE3-EGFP. The result shown is a representative FRAP experiment with initial fluorescence intensity before photobleaching set at 100%. Data were collected as 50 images every 9 seconds. M_f=50.5±4.6% and D_eff=(3.9±1.2) x10^–10 cm²/second, for six cells in two separate studies on both untreated and LY294002-treated cells. Data shown are mean±s.e.m.