Fig. 2. Behavior of actin mutants on native gels. The ³⁵S-labeled products of the in vitro translation assays were run on native polyacrylamide gels with or without ATP and imaged using autoradiography. On the native gel with ATP, G182D stuck to CAP and both mutants L94P and E259V remained bound to CCT and prefoldin, and showed an absence of folded actin. The 16 other mutants were folded correctly, as indicated by an abundance of folded actin that was similar to the wild type. On the gel without ATP, G15R, N115S, V163L and G182D showed an increase in binding to CAP. Representative gels shown from three experiments performed.