Fig. 3. Detection of myeloid DC cell surface-associated transcripts in IL-4-DC by RT-PCR. Cells were induced to mature for 48 hours with TNF- (20 ng/ml). Total RNA was extracted and analysed for expression of mRNA for CD1a, CD1b, CD1c, CD83, HLA-DR, CCR7, CXCR4 and ß-actin. Data shown are representative of four similar experiments.