Fig. 7. Immunostimulatory functions of immature, and mature, IL-4-DC and GM-IL-4-DC. MLR was performed with both immature (A) and mature (B,C) stimulator cells as shown. Maturation of stimulator cells was induced either by TNF- (B) or CD40 ligation (C). Proliferation was measured by [³H]thymidine incorporation (cpm). The background proliferation of T cells alone was always less than 350 cpm. The data represent mean ± s.d. of triplicate wells. One representative experiment of three is shown.