Fig. 2. Light microscopy of retinas from wild-type and mutant mice whose eyes were prepared by `standard' means (i.e. imposing mechanical stress on the retina). (A-D) H&E staining reveals a normal laminar structure in wild-type (wt) and GFAP^–/– (g–/–) retinas, partial separation of the ILM in a Vim^–/– (v–/–) retina, and complete separation in a GFAP^–/– Vim^–/– (g–/–v–/–) retina. Immunohistochemical visualization of ILM by laminin antibodies (E,F), astroglial cells by S100 antibodies (G,H) and neuronal processes in the ganglion cell layer (GCL) and outer plexiform layer by antibodies against neurofilament-M (I,J, green) and the distribution of retinoschisin (Rs1) (I,J, red) in wild-type and GFAP^–/– Vim^–/– retinas. The separated layer in GFAP^–/– Vim^–/– mice consists of the ILM (F,H, arrows) and parts of Müller cells (H). Arrows indicate the ILM and asterisks indicate separation between the ILM and the rest of the retina. Abbreviations: INL, inner nuclear layer; ONL, outer nuclear layer. Scale bars, 50 µm (A-D), 20 µm (E,F), 50 µm (G-J).