Fig. 2. CD133⁺ cells are restricted to the ₂ß₁^hi population and are largely quiescent. Basal cells were selected for ₂ß₁^hi and ₂ß₁^low expression. (A) ₂ß₁^hi population labelled with antibodies to CD133 (red) and basal cell marker 34ßE12 (green). Co-localization (yellow/orange; yellow arrow). Scale bar: 5 µm. (B,C) Graph of ₂ß₁^hi population (B) and ₂ß₁^low population (C) labelled with anti-CD133 antibody (shaded area) and analysed by flow cytometry. Isotype control is depicted as a solid black line. (D) Dot-plot showing flow cytometric analysis of basal cells double-labelled with anti-CD133 (APC) and Ki-67 (FITC) from a representative experiment (n=3).