Fig. 5. Effect of SPANs on the distribution of cytoskeletal markers. Untreated CGNs (A-C) were compared with CGNs treated for 1 hour with ß-BTx (D-F). The distribution of F-actin (A,D) and of neuron-specific neurofilaments (NF) (B,E) changed from filamentous structures to dotted aggregates, whereas the appearance of tubulin (C,F) remained unaltered. Scale bar, 50 µm.