Fig. 8. SPANs induce SV exocytosis without proportionate recycling. Control CGNs (A) and cells treated for 1 hour at 37°C with notexin (B), ß-BTx (C), textilotoxin (D) or taipoxin (E) were incubated for 5 minutes at 37°C with anti-SytI-ecto antibodies before fixation and then processed for indirect immunofluorescence. (F) Higher magnification of the area indicated by the square in (E), which highlights the bright selective staining of bulges in the intoxicated sample (arrows). Scale bar, 50 µm (A-E).