Fig. 8. Effect of troglitazone on the stretch-induced inhibition of adipocyte differentiation of 3T3-L1 cells. (A) Phase-contrast microscopic images Oil-Red-O-stained 3T3-L1 cells after a maturation period of 9 days. (a) 3T3-L1 cells without induction (Pre). (b) Unstretched cells (Rest) incubated with 0.01% DMSO (Vehi) during the induction period. (c) Unstretched cells incubated with 30 µM troglitazone (Tro) during the induction period. (d) Stretched cells (Stretch) incubated with 0.01% DMSO (Vehi) during the induction period. (e) Stretched cells incubated with 30 µM troglitazone during the induction period. (B) GPDH activity and (C) intracellular triglyceride accumulation after maturation period of differentiated 3T3-L1 cells with (grey) or without (black) cyclic stretching (130%, 1 Hz), and in the presence or absence of troglitazone (30 µM) during the induction period (n=5 each). Abbreviations: Tro, 30 µM troglitazone; Vehi, 0.01% DMSO; DEX/MIX/INS, induction medium containing DEX, MIX, INS and FBS; ^*P<0.05; ^**P<0.01.