Fig. 7. Atomic force micrographs of F-actin. Actin (5 µM) was incubated in polymerization buffer in absence (A,B) or presence of EhCaBP1 (C,D). The samples were diluted 1:5000 times before mounting on freshly cleaved mica for atomic force microscopy. (A,C) two-dimensional view; 2 µm scan. (B,D) Three-dimensional top view; 0.8 µm scan.