Fig. 2. Colocalisation of lumFP, COPII and ERGIC-53 at 15°C. Cells were transfected with plasmid DNA encoding lumYFP and incubated at 15°C for 2 hours prior to fixation, processing for immunofluorescence and imaging. lumYFP (A,D) colocalises with COPII (Sec13p) (B) and ERGIC-53 (E) (arrowheads). C and F are merged images of the respective images in the same row. Bars, 10 µm.