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Fig. 1. Testes isolated from P-element-mediated mutants have diminished Klp67A levels. Western blot showing the different allelic combinations used in this study. A band of the expected molecular weight of ~92 kDa was present in Oregon R testes but was greatly diminished in homozygous mutant third instar larvae and not detectable in homozygous pharate adults or hemizygous or heteroallelic individuals at any stage. {alpha}-tubulin is shown as a loading control.