Fig. 5. Detection of KIN17 protein in adult mouse testis after nuclease digestion. Immunohistochemical detection of KIN17 protein (D,G,J,M) in testicular sections digested either with DNase I (E,F,G), or with RNase A (H,I,J), or in a combination of the two (K,L,M). DAPI staining (A, control) shows that DNase I digestion released DNA from nuclei of testicular cells, except elongated spermatid heads (E) and that DNA is preserved by RNase A digestion (H). PI staining shows that DNA and RNA are released from nuclei by DNase and RNase digestion (K). Whatever the treatment, a residual staining for KIN17 is observed in cell nuclei; cytoplasmic staining of elongating spermatids is well preserved (arrowheads in D, G, J and M) compared to control (D). SC35 detection is not affected by DNase I digestion (F) compared to control (B) and RAD 51 is not affected by RNase digestion (I) compared to control (C). A negative control where anti-KIN17 mAb was preadsorbed with pure KIN17 protein is shown (L). Ser, Sertoli cells; S, preleptotene, leptotene; Z, zygotene; P, pachytene spermatocytes; Rs, round spermatides; Es, elongated spermatids. Bars: A,E,H, 80 µm; B,C,D,F,G,I, 22 µm; J,L,M, 23 µm; K, 55 µm.