Fig. 3. Disruption of focal adhesions in myoblasts treated with MIs. Asynchronous myoblasts were incubated for 24 hours in 2% HS + DMSO, 30 mM BDM or 15 µM ML7. (A) Cells were triple-stained to reveal actin (green), the focal adhesion component vinculin (red) and DNA (blue). Vinculin-positive focal adhesions were located at the ends of stress fibers in control cells. Stress fibers and focal adhesions were disrupted in MI-treated cells, accompanied by an increase in diffuse cytoplasmic staining. Scale bar: 20 µm. (B) Immunodetection of the focal adhesion component paxillin (red). Both the number and size of paxillin⁺ focal contacts were reduced by MI treatment. Scale bar: 10 µm.