Fig. 6. MI treatment inhibits differentiation. Dense asynchronous cultures were treated with 2% HS containing DMSO (control), 15 mM BDM or 15 µM ML7 for 3 days, and stained for muscle-specific myosin expression. Control cultures fused into large multinucleated myotubes, but BDM and ML7 inhibited fusion. Scale bar: 25 µm.