Fig. 2. Mutant GFP-PfVps4 localizes to punctuate structures when transiently expressed in P. falciparum. (A) Cartoon illustrating the general domain structure of the GFP-PfVps4 chimera and the mutations predicted to block ATP binding or ATP hydrolysis. (B) Pattern of GFP fluorescence in stable line of P. falciparum expressing GFP-PfVps4-WT. Corresponding phase contrast images are illustrated. (C) Pattern of GFP fluorescence in P. falciparum transiently transfected with GFP-PfVps4-E214Q. Arrows indicate the punctuate structures.