Fig. 6. LY294002 (LY) and wortmannin (WT) affect the extent of cleavage of dividing crane fly spermatocyte cells and the effect of WT is reversible. Bars with dots, average maximum diameters in µm (at the start of cleavage). Clear bars, average minimum diameter at the end of cleavage in control cells (n=8) and at the end of treatment in cells treated with LY (n=7) or WT (n=8), as indicated. Dark gray bars, average diameter after washing out WT (n=4). After washout of WT cytokinesis resumes and the diameters become smaller. Error bars represent s.d. Minimum diameters of control cells were not significantly different from those of washed out WT-treated cells (P=0.3, Student's t-test).