Fig. 3. Clp1p is important for the maintenance of the actomyosin ring at the medial region of the cell. (A) cps1-191 and clp1cps1-191 were grown to exponential phase at 24°C, shifted to 36°C for four hours and stained with rhodamine-conjugated phalloidin to determine the distribution of actin. `Medial' refers to the localization of actin in a medial actomyosin ring structure. `Tip' refers to the localization of actin patches to the cell ends. (B) Wild-type and clp1 cells were cultured to exponential growth phase at 24°C, synchronized in early G2 by centrifugal elutriation and then treated with a low dose (0.2 µM) of Lat A or DMSO (solvent control). Cells were subsequently fixed at 30-minute intervals and stained with DAPI (nuclei) and ALEXA-488 conjugated phalloidin (actin). The graph shows the percent cells with actomyosin rings. (C) Images of cells treated as in B.