Fig. 1. Morphological changes in ACHN cells after treatment with the Stx1-B subunit. ACHN cells stained with Cell-tracker Green were treated with and without 5 µg/ml of the Stx1-B subunit for the indicated periods and visualized using confocal microscopy. Filopodia- and lamellipodia-like structures are visible at higher magnifications and are indicated by the arrowheads. Results are representative of three independent experiments.