Fig. 5. Effect of Stx1-B subunit on the distribution of vimentin and cytokeratin in ACHN cells. ACHN cells were examined as described in Fig. 2 using Alexa-488-labeled anti-vimentin mAb (left panels, green) and Alexa-546-labeled anti-cytokeratin mAb (center panels, red). The arrowheads indicate the perinuclear clustering of vimentin. Results are representative of three independent experiments.