Fig. 9. Effect of inhibitors on the Stx1-B-subunit-mediated phosphorylation of ezrin. ACHN cells were preincubated with the inhibitors shown in the figure for three hours. The concentrations of 300 and 900 µM for MBD and 25 and 100 µM for other inhibitors were used. After treatment with the Stx1-B subunit for 5 minutes, cell extracts were prepared, and immunoblotting for phospho-specific ezrin was performed as described in Fig. 8. In parallel, each sample was examined using an anti-ezrin antibody to confirm that the protein amounts in each lane were comparable (only the result for PP2 is shown in the second panel from the top). In the right panel, the effect of each inhibitor is schematically presented.