Fig. 3. Distribution of phosphorylated proteins during bacterial entry. Non-infected cells (top) and cells infected for 5 minutes with FITC-coupled C. caviae GPIC (bottom) were labelled with anti-phosphotyrosine antibody (middle) while actin was visualized using Alexa546-coupled phalloidin (left). Upon infection, phosphorylated proteins were concentrated at the sites of intense actin polymerization, where bacteria are found (arrows).