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Fig. 4. Localization of TRF2, B23 and UBF in prophase and metaphase. The immunofluorescence of TRF2 and B23 or UBF is shown in mitotic human MCF-7 cells during prophase (A-C and H-K) and metaphase (D-G and L-O). Panels A-C were observed by light microscopy and panels D-O were observed by laser-scanning confocal microscopy. Panels G', K' and O' depict the fluorescent measurements along the indicated lines in G, K and O, respectively.