Fig. 2. Ultrastructure of peroxisomes in (A) controls and (B,C) after silencing of DLP1. HepG2 cells were transfected with buffer (Con) (A) or with DLP1 siRNA duplexes (B,C). After 48 hours the cells were processed for cytochemical visualization of peroxisomal catalase and embedded for electron microscopy. (A) Typical single, spherical peroxisomes (Po). (B,C) Peroxisomes after silencing of DLP1. They are arranged like beads on a string and are constricted but still interconnected (arrows). Bars, 500 nm.