Fig. 4. Perturbation of peroxisome morphology by Pex11pß expression after silencing of DLP1. COS-7 cells stably expressing GFP-PTS1 (GFP) were transfected with (A) buffer (Con) or (B-E) DLP1 siRNA duplexes (siRNA), incubated for 24 hours, transfected with a Pex11pß-myc expression vector and another dose of siRNA duplexes, and processed for immunofluorescence after an additional 24 hours. (A,B) Pex11pß-myc staining with antibodies to the myc epitope tag. (C-E) GFP-PTS1 labeling of peroxisomes in Pex11pß-myc-expressing cells. (D) Higher magnification image of boxed region in C. Note the extreme tubulation of peroxisomes (arrows) in cells treated with DLP1 siRNA/Pex11pß-myc, and the absence of peroxisome segmentation. N, nucleus. Bars, 10 µm.