Fig. 1. TLR2 is present in lipid rafts following LTA stimulation. Monocytes were either not stimulated (A,B) or stimulated with 100 mg/ml LTA in 5% HPS for 30 minutes (C) prior to solubilisation with 1% Triton X-100 buffer for 1 hour on ice and then subjected to sucrose density gradient centrifugation. Fractions were collected from the top of the gradient and equivalent portions of each fraction were analysed by SDS-PAGE and immunoblotting. The lipid raft marker was detected using HRP-conjugated cholera toxin (A), the nitrocellulose membranes were also probed with TLR2 specific mAb (B,C). The relative positions of the raft and non-raft (soluble) fractions are indicated.