Fig. 3. Coexpression of AFP and albumin in cultured rat small hepatocytes. Cells were cultured for 8 weeks, fixed with 4% paraformaldehyde in the presence of Triton X-100 and subsequently processed for immunofluorescence localization of AFP (a) and albumin (b). Typical cells coexpressing AFP and albumin are indicated by arrow heads. (c) Phase-contrast microscopy picture. Scale bar, 40 µm.