Fig. 3. Anisomycin blocks the calcium leak from intracellular stores induced by puromycin. (A,B) The intracellular stores of LNCaP cells were loaded with the calcium indicator mag-fluo-4. Recordings of the leak from LNCaP cells, permeabilized with digitonin in two representative LNCaP cells in response to the application of puromycin are shown. Addition of puromycin induced a slow reduction in [Ca²⁺]_ER (A) whereas anisomycin pre-treatment inhibits passive Ca²⁺ leakage through the ER in response to puromycin application (B). (C) Cumulative data (mean±s.e.m.) for control and anisomycin-treated LNCaP cells of percentage of calcium released from internal stores.