Fig. 5. Akt activity in reconstituted human epidermis. (A) Western blots probed with anti-ER or anti-phospho-Akt⁴⁷³ antibodies. Cells were transduced with empty retroviral vector (pBabe), myrAktER or A2myrAktER and treated with 4OHT for 24 hours. (B-I) Epidermis reconstituted by keratinocytes transduced with GFP (B,C), FOXN1ER (D,E), myrAktER (F,G) or A2myrAktER (H,I). Sections were subject to immunofluorescent staining using antibodies to the oestrogen receptor (B,D,F,H) or phospho-Akt⁴⁷³ (C,E,G,I) (green). Propidium iodide was used as a nuclear counterstain (red). Scale bar: 50 µm.