Fig. 7. Purification of Cpc1 by Chlamydomonas flagellar fractionation. (A,B) 6% SDS polyacrylamide gel (A) and corresponding western blot (B) of wild-type axonemes (Ax), the insoluble material following successive extractions with 0.5 M NaCl (NP) and 0.2 M KI (KP) and the material solubilized by KI (KS). Western blotting with anti-Cpc1A shows that Cpc1 is extracted by KI. Stoichiometric amounts were loaded in each lane. Dots in (A) mark the location of high molecular weight central pair proteins CP3 and CP4.