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Fig. 4. Concentration-dependent effects of ethanol on the localization of Rat8p and export of bulk poly(A)+ mRNA. Yeast cells in exponential phase were treated with ethanol (6-9% v/v) for 15 minutes and the localization of GFP-Rat8p was monitored. Cells at each ethanol concentration were also fixed for in situ hybridization using Cy3-labeled SSA4 probes to detect poly(A)+ RNA and DAPI staining.