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Fig. 6. Overexpression of Rat8p attenuated the blocking of export of bulk poly(A)+ mRNA in ethanol stressed cells. (A) Yeast cells overexpressing GFP-Rat8p (W303-1A with pRS426-GFP-RAT8) were treated with 10% ethanol for 15 minutes. GFP-Rat8p fluorescence was detected before (w/o stress) and after (10% EtOH) treatment. (B) Cells overexpressing Rat8p (W303-1A with pRS426-RAT8) were treated with 10% ethanol for 15 minutes and localization of bulk poly(A)+ mRNA was monitored before and after treatment. Cells carrying the empty vector (pRS426) were used as a control. Cell nuclei were stained with DAPI.