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Fig. 3. SNX16 associates with EGFR. (A) COS-7 cells were transfected with FLAG-tagged SNX16 and treated with EGF for the indicated times. Cell lysates were immunoprecipitated with anti-FLAG antibody and then immunoblotted with anti-EGFR antibody. Total cell lysates (40 µg) were then immunoblotted with anti-EGFR and anti-FLAG antibody. (B) COS-7 cells were transfected with GFP-tagged SNX16 and treated with EGF for the indicated times. Fixed cells were processed to simultaneously detect SNX16 (green) and EGFR (red) by confocal microscopy. Bars, 10 µm.