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Fig. 6. The PX domain is required for the homo-oligomerization of SNX16. Various HA or FLAG-tagged SNX mutants were coexpressed in COS-7 cells. FLAG-tagged proteins were immunoprecipitated and analyzed by immunoblotting using anti-HA antibody. Total cell lysates (40 µg) were then immunoblotted with anti-HA and anti-FLAG antibody.