Fig. 5. Peptide-MHC class II complexes derived from endogenous transmembrane or cytosolic protein are localized to lysosomal compartments in bone marrow-derived dendritic cells. GM-CSF cultured BM-DCs from B10A.(5R) (A) or C57B1/6 (B) mice were either directly fixed (A) or first pulsed cytosolically with GST-E_52-68-myc protein, chased, fixed and then stained for Y-Ae, anti-LAMP-1 antibodies. Images are single representative confocal sections. All images on the very right are respective overlays of the stained images of A and B. Scale bar, 10 µm.