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Fig. 5. Alfy is ubiquitously expressed. Extracts from cell lines (A) or mouse tissues (B) were subjected to 6% SDS-PAGE and immunoblotted using rabbit anti-Alfy antibodies. The asterisk in (A) indicates an unidentified protein in cell lines recognized by the anti-Alfy antibody. Abbreviations: B, brain; H, heart; K, kidney; L, lung; M, skeletal muscle; P, pancreas; S, spleen.