Fig. 6. Alfy colocalizes with hAtg5 and LC3 by confocal microscopy. HeLa cells grown on coverslips were starved for 45 minutes in HBSS, permeabilized, fixed and double stained against endogenous Alfy (A,D,G,J) and endogenous EEA1 (B), endogenous LAMP-1 (E), overexpressed GFP-hAtg5 (H) or GFP-LC3 (K). Yellow colour in the merged images (C,F,I,L) indicates colocalization. Colocalization between Alfy and autophagic markers is indicated by arrows. Scale bar, 10 µm.